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1. Sonication Help! - (reply: 3)
2. ChIP for a transcriptional coactivator - (reply: 1)
3. Can local chromatin structure affect sonication? - (reply: 3)
4. Trouble validating in multiple cell types - (reply: 1)
5. histone chip-seq profiles look similar to input! - (reply: 4)
6. isolating whole chromatin before ChIP - (reply: 1)
7. Software to align sequences - (reply: 1)
8. ChIP assay - about input - (reply: 1)
9. Quantify Chromatin With Protein Assay? - (reply: 1)
10. ChIP troubleshooting - controls for each step? - (reply: 3)
11. How many ug of DNA per ChIP is recommendable. - (reply: 5)
12. ChIP sonication - (reply: 4)
13. Ab for acetyl modifications in low-input ChIP experiments - (reply: 1)
14. abcam 209 GFP antibody for CHIP- is there an alternative GFP antibody useful for - (reply: 5)
15. no DNA fragment pull down in the first CpG island (MBD-seq) - (reply: 2)
16. Quantify DNA before ChIP PCR - (reply: 3)
17. ChIP input - sonication problem? - (reply: 6)
18. Sequential ChIP with a repeat of the sonication step? - (reply: 1)
19. ChIP kit recommendations - (reply: 3)
20. What remains after elution? - (reply: 1)
21. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
22. Sonication woes for Chip-seq, using Bioruptor - (reply: 5)
23. Sonication problem with BOTH BioRuptor and Covaris - (reply: 9)
24. Can I go for ChP having frozen tissue - (reply: 4)
25. help-Chip antibody p65 ,NFKB mouse - (reply: 4)
26. Shearing issue: always having unsheared DNA (Please help!) - (reply: 9)
27. Input reverse cross linking - (reply: 1)
28. questions about shearing chromatin analysis - (reply: 3)
29. Advice on sonication (c2c12) - (reply: 12)
30. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
2. ChIP for a transcriptional coactivator - (reply: 1)
3. Can local chromatin structure affect sonication? - (reply: 3)
4. Trouble validating in multiple cell types - (reply: 1)
5. histone chip-seq profiles look similar to input! - (reply: 4)
6. isolating whole chromatin before ChIP - (reply: 1)
7. Software to align sequences - (reply: 1)
8. ChIP assay - about input - (reply: 1)
9. Quantify Chromatin With Protein Assay? - (reply: 1)
10. ChIP troubleshooting - controls for each step? - (reply: 3)
11. How many ug of DNA per ChIP is recommendable. - (reply: 5)
12. ChIP sonication - (reply: 4)
13. Ab for acetyl modifications in low-input ChIP experiments - (reply: 1)
14. abcam 209 GFP antibody for CHIP- is there an alternative GFP antibody useful for - (reply: 5)
15. no DNA fragment pull down in the first CpG island (MBD-seq) - (reply: 2)
16. Quantify DNA before ChIP PCR - (reply: 3)
17. ChIP input - sonication problem? - (reply: 6)
18. Sequential ChIP with a repeat of the sonication step? - (reply: 1)
19. ChIP kit recommendations - (reply: 3)
20. What remains after elution? - (reply: 1)
21. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
22. Sonication woes for Chip-seq, using Bioruptor - (reply: 5)
23. Sonication problem with BOTH BioRuptor and Covaris - (reply: 9)
24. Can I go for ChP having frozen tissue - (reply: 4)
25. help-Chip antibody p65 ,NFKB mouse - (reply: 4)
26. Shearing issue: always having unsheared DNA (Please help!) - (reply: 9)
27. Input reverse cross linking - (reply: 1)
28. questions about shearing chromatin analysis - (reply: 3)
29. Advice on sonication (c2c12) - (reply: 12)
30. Normalizing to negative control primers for ChIP qPCR - (reply: 3)