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1. Shearing issue: always having unsheared DNA (Please help!) - (reply: 9)
2. Input reverse cross linking - (reply: 1)
3. questions about shearing chromatin analysis - (reply: 3)
4. Advice on sonication (c2c12) - (reply: 5)
5. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
6. Neeeeed Help with my CHIP! Input value so high~ - (reply: 5)
7. Matrix ChIP - No signal - (reply: 9)
8. I forgot treat my samples with proteinase K before the DNA extraction step - (reply: 1)
9. Some ChIP Questions - (reply: 4)
10. Quenching the crosslinking - (reply: 2)
11. RNA immunoprecipitation DNase treatment - (reply: 4)
12. Formaldehyde cross linking problem!!! - (reply: 2)
13. I'd like your advice on NIH 3T3 sonication - (reply: 2)
14. ChIP data normalization - (reply: 2)
15. help on % input calculation please!! - (reply: 1)
16. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
17. ChIP on episomal DNA - (reply: 2)
18. ChIP amplification problems - (reply: 1)
19. Highly Variable DNA yield from Neutrophils lysed & sheared for ChIP - (reply: 7)
20. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
21. ChIP from mouse tissues with antibody raised in mouse - (reply: 1)
22. Washing buffer and use of Protease inhibitors - (reply: 1)
23. SDS Precipitation - (reply: 4)
24. Proteinase K step - (reply: 2)
25. Cell lysis before shearing - (reply: 5)
26. ChIP-Seq with HA-tagged protein - (reply: 1)
27. ChIP-qPCR gives odd amplification plot - (reply: 5)
28. ChIP help- Ab from different sources - (reply: 2)
29. chip precipitation - using isopropanol in chip precipitation (reply: 1)
30. Is my ChIP result positive or negative - unusual ChIP result. (reply: 4)
2. Input reverse cross linking - (reply: 1)
3. questions about shearing chromatin analysis - (reply: 3)
4. Advice on sonication (c2c12) - (reply: 5)
5. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
6. Neeeeed Help with my CHIP! Input value so high~ - (reply: 5)
7. Matrix ChIP - No signal - (reply: 9)
8. I forgot treat my samples with proteinase K before the DNA extraction step - (reply: 1)
9. Some ChIP Questions - (reply: 4)
10. Quenching the crosslinking - (reply: 2)
11. RNA immunoprecipitation DNase treatment - (reply: 4)
12. Formaldehyde cross linking problem!!! - (reply: 2)
13. I'd like your advice on NIH 3T3 sonication - (reply: 2)
14. ChIP data normalization - (reply: 2)
15. help on % input calculation please!! - (reply: 1)
16. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
17. ChIP on episomal DNA - (reply: 2)
18. ChIP amplification problems - (reply: 1)
19. Highly Variable DNA yield from Neutrophils lysed & sheared for ChIP - (reply: 7)
20. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
21. ChIP from mouse tissues with antibody raised in mouse - (reply: 1)
22. Washing buffer and use of Protease inhibitors - (reply: 1)
23. SDS Precipitation - (reply: 4)
24. Proteinase K step - (reply: 2)
25. Cell lysis before shearing - (reply: 5)
26. ChIP-Seq with HA-tagged protein - (reply: 1)
27. ChIP-qPCR gives odd amplification plot - (reply: 5)
28. ChIP help- Ab from different sources - (reply: 2)
29. chip precipitation - using isopropanol in chip precipitation (reply: 1)
30. Is my ChIP result positive or negative - unusual ChIP result. (reply: 4)