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1. Help !! How to induce early apoptosis without using chemical agents in c - (reply: 2)
2. Does this qualify as a whole cell-based assay? - (reply: 1)
3. Check cell viablity after FACS - (reply: 1)
4. Luciferase assay-normalisation - (reply: 2)
5. how to find the correct receptor to my ligand among many possible ones - (reply: 4)
6. quantify immunofluorescence staining - (reply: 1)
7. Storage of tissue at -80 followed by a breif unthaw prior to extraction - (reply: 1)
8. how to select ki67 antibody - (reply: 1)
9. cell population doubling - (reply: 1)
10. temperature tolerance of animal cells - (reply: 2)
11. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
12. Need help with clonal isolation - (reply: 1)
13. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
14. Problem of MTT Solvent (10% SDS in 0.01M HCl) for dissolving formazan crystals - (reply: 1)
15. Fugene 6 + serum free DMEM + problem - (reply: 2)
16. normalization to protein content or cell number - (reply: 2)
17. MCF10A and MCF12A - (reply: 2)
18. Help with Cellular Fractionation - (reply: 1)
19. a big problem with INVITROGEN Caspase-3 colorimetrik kit - (reply: 3)
20. DiI staining Fixation protocol - (reply: 1)
21. PARP cleavage kinetics during apoptosis - (reply: 3)
22. Help with Dual luciferase assay - (reply: 1)
23. Neutrophil cell lines and plasma cell lines of choice - (reply: 1)
24. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
25. Nucleus fills the whole cell - (reply: 3)
26. Detach cells and stop stimulation simultaneously- How? - (reply: 4)
27. Tetrapotassium vs tetrasodium salt - (reply: 2)
28. Carrier protein concentration - (reply: 2)
29. Advise on cellular fluorescent assay - (reply: 2)
30. Calculating the amount of the desired cells in the medium ? - (reply: 3)
2. Does this qualify as a whole cell-based assay? - (reply: 1)
3. Check cell viablity after FACS - (reply: 1)
4. Luciferase assay-normalisation - (reply: 2)
5. how to find the correct receptor to my ligand among many possible ones - (reply: 4)
6. quantify immunofluorescence staining - (reply: 1)
7. Storage of tissue at -80 followed by a breif unthaw prior to extraction - (reply: 1)
8. how to select ki67 antibody - (reply: 1)
9. cell population doubling - (reply: 1)
10. temperature tolerance of animal cells - (reply: 2)
11. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
12. Need help with clonal isolation - (reply: 1)
13. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
14. Problem of MTT Solvent (10% SDS in 0.01M HCl) for dissolving formazan crystals - (reply: 1)
15. Fugene 6 + serum free DMEM + problem - (reply: 2)
16. normalization to protein content or cell number - (reply: 2)
17. MCF10A and MCF12A - (reply: 2)
18. Help with Cellular Fractionation - (reply: 1)
19. a big problem with INVITROGEN Caspase-3 colorimetrik kit - (reply: 3)
20. DiI staining Fixation protocol - (reply: 1)
21. PARP cleavage kinetics during apoptosis - (reply: 3)
22. Help with Dual luciferase assay - (reply: 1)
23. Neutrophil cell lines and plasma cell lines of choice - (reply: 1)
24. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
25. Nucleus fills the whole cell - (reply: 3)
26. Detach cells and stop stimulation simultaneously- How? - (reply: 4)
27. Tetrapotassium vs tetrasodium salt - (reply: 2)
28. Carrier protein concentration - (reply: 2)
29. Advise on cellular fluorescent assay - (reply: 2)
30. Calculating the amount of the desired cells in the medium ? - (reply: 3)